![]() Not all locations will accept your kit because it is a biohazard. While I was waiting roughly an hour to get my blood spun, I googled which FedExs and UPSs near me would accept my kit. Even if you have to find a lab to draw/spin your blood, you still have to find a doctor/nurse practitioner (or Terri Warren) who will sign off on your kit! You can't go to the lab without a doctor or nurse practitioner signing the provider's form that comes with the kit. Any Lab Test Now is popular in my region and will draw and spin your blood and ship your kit back to the University of Washington for $120. Though if your doctor can't, you can have the folks at the University of Washington help you in finding a lab close to you that will. My doctor was able to do that at her office. You have to get your blood drawn and spun for the kit. But you can find out on the Westover Heights website. I do know you'll have to make an appointment and pay her for that as well, though I'm not sure how much. If your doctor does not support you getting the Western Blot, you can get in contact with Terri Warren at Westover Heights and she'll sign off on it for you. I told my doctor about the test because you need a doctor to sign off on it and she gave me no issues. Fill out whatever you can fill out on the forms that are given to you within the kit. I put the bag containing the test tubes in the fridge just to be safe. Once you get your kit, put the icepack that it came with in the freezer. The kit arrived in roughly 3 business days, shipping across the country. You don't pay for the kit then and you don't pay for shipping either. I called the University of Washington to order a Western Blot kit over the phone. To my surprise, after I explained everything to him, he was very supportive! He even offered to pay for my test after hearing how expensive it was, which I politely declined. I explained the test to him and the probabilities we'd be faced with (with info from Terri Warren from Westover Heights). I had already looked up the Western Blot the first time I got a positive HSV2 result back but I didn't go through with it because I didn't have the money and I wasn't with anyone anyway at the time. I immediately felt so fearful and anxious because I knew I had to disclose that information to the guy I was dating and things were going so well and I didn't know how he was going to take it. ![]() To my surprise I ended up with another low positive for HSV2 after testing negative. I ended up meeting this guy in February and we dated for a bit and decided to get tested (in March) before things got physical. I thought by some miracle my low positives were false and proceeded to move on with my life accordingly. Had to wait until I got private insurance again before going back) and I tested negative for HSV2. I ended up getting tested for the full STD panel by my same doctor in July of last year (got kicked off my parent's insurance and was on Medicaid while I was in grad school and my doc didn't take it. At that point I accepted the fact that I had genital herpes even though I never had any symptoms. The test came back with another low positive of 1.67 and another negative supplement. My supplement was negative so my doctor retested me after waiting roughly a month. All rights reserved.So originally I (27F) had tested positive for HSV2 in 2020 with a low positive IgG value of 1.43. Modifications of the cutoff points of the ELISA test were obtained with technical adjustments done to detect HSV-2 antibodies by ELISA and Western blot using DBS samples.ĭried blood spots ELISA Herpes simplex virus type 2 Western blot.Ĭopyright © 2018 The Authors. A 1:5 dilution was used and the incubation times were increased for the Western blot.ĩ08 DBS samples were processed and the following cutoff points were determined: negative (0-3.79), undetermined (3.8-4.6) and positive (≥4.61), with sensitivity and specificity close to 95%. ![]() ELISA was performed with 100μl and the Western blot with 200μl of eluted DBS. Samples were processed by both methods to determine ELISA cutoff points using ROC curves. IgG-G2 ELISA (Human ® Diagnostics, Germany) and Western blot IgG/IgM (EUROLINE-WB, Euroimmun ® Germany) tests were modified to use DBS samples. The objective of current study was to evaluate the use of DBS samples to detect HSV-2 antibodies using commercial ELISA and Western blot tests. HSV-2 is the main cause of genital ulcers and is diagnosed mainly with serological tests. Herpes simplex virus type 2 (HSV-2) is a sexually transmitted agent and is detected worldwide. ![]()
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